Project #3

2019-11-06 07:29

"Scientific Research Project"

This project is an individual scientific research work sponsored by Freshmen Research Initiatives and the College of Natural Science Department. The topic of the project is "Developing a Preventive Mechanism for Rhinovirus-C Infection." Human rhinovirus is a virus that causes common cold illnesses, associated with upper respiratory tract infection. Rhinovirus C (RV-C), one of the three genetically distinct groups of rhinovirus, is mainly responsible for more severe illnesses such as asthma and pneumonia, especially among children. On a molecular level, the expression of human protein Cadherin-related facility member (CDHR3), more specifically, its structural domain 1 and 2 are thought to be the binding sites for RV-C that is then introduced in our body as its expression is positively correlated with RV-C binding and replication. To further analyze the protein structure and its mechanism, purification can be done. However, CDHR3 is a cell membrane receptor protein that remains in the cell debris due to its insolubility, preventing it from being purified. Furthermore, CDHR3 could not only bind to RV-C and may have different functions. Therefore, site-directed mutagenesis was introduced on a target site of the human receptor protein gene and then its truncation mutant was created via Gibson Assembly to further purify and develop antibodies against the receptor to inhibit Rhinovirus infection.